TM 12 Genetic engineering in action 1 (Biologi Molekuler ).pptx – Download as .ppt /.pptx), PDF File .pdf), Text File .txt) or view presentation slides online . Perkembangan Biologi Molekuler dan Pembelajarannya. Sutiman B. Sumitro. Laboratorium Biologi Sel dan Molekuler. Fakultas MIPA. Universitas Brawijaya. Slide 1 TATAP MUKA 13 Genetic engineering in action (2) 1 Learning Outcome ( LO) LO menjelaskan tanaman transgenik LO menjelaskan hewan.

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Daerah non-esensial blok hitam dapat dimanipulasi dalam konstruksi vektor. This talk will focus on the links and controls of the onset of the cell cycle and apoptosis.

BIOLOGI MOLEKULER Pengenalan Biologi Molekuler Materi Genetik

An important plasmid in the history of gene manipulation is pBR, which was developed by Francisco Bolivar and his colleagues. Given the problems in achieving correct expression and processing of some mammalian proteins in non-mammalian hosts, this is proving to be an important development of transgenic animal technology.

Kedua plasmid saling melengkapi secara trans untuk memungkinkan transfer DNA kloning ke dalam genom tanaman. When chromosomes are replicated during cell division, a stretch of the telomere is left unreplicatedmaking the telomere a bit shorter with each division. The resulting fertilised eggs were implanted into the uteri of foster mothers, and some of the mice resulting from this expressed the GH gene. Such changes are often aimed at improving the productivity and efficiency of crop plants, both of which are important to help feed and clothe the increasing world population.

About project SlidePlayer Terms of Service. In addition, the pBR series has been the basis for the development of many more plasmid vectors with additional features. Protecting the vital DNA molecule from being copied out of synch, these telomeres provide a kind of buffer zone where asynchronous replication errors that are inevitable will not result in any of the vital DNA sequences being lost.

This region is known as a polylinker or multiple cloning site MCSand is useful because of the choice of site available for molejuler of DNA fragments during recombinant production.

Hal ini sebagian disebabkan oleh ukuran dan kompleksitas genom, dan sebagian disebabkan oleh kenyataan bahwa pengembangan tanaman dan hewan adalah proses yang sangat kompleks yang masih belum spide dipahami pada tingkat molekuler. The organism is amenable to classical genetic analysis, and a range of mutant cell types is available.


Basically a chromosome is one long chain of genetic material. One of the first recombinant DNA experiments to be performed with plants did not in fact produce transgenic plants at all butinvolved the use of genetically modified bacteria. Fortunately the methods available provide a relatively simple way to isolate specific gene sequences. To use this website, you must agree to our Privacy Policyincluding cookie zlide. Primary way scientists categorize and classify viruses.

The cells can then be plated out onto selective media for propagation of cells harbouring the plasmidtransformation frequencies of around or transformants per microgram are more often achieved in practice. Thus, the dark areas in the fore- and hindbrain are regions where the lacZ gene has been expressed. Phage DNA and packaging.

The plural term phages is used when different types of phage are being considered; we therefore talk of T4, M13 and as being buologi. By over half of the soybean crop in the USA was planted with Bt-engineered plants, although there have been slive problems with pests milekuler resistance to the Bt toxin. The vector M13mp19 is identical except for the orientation of the polylinker region. The plasmid has all the features of a good vector, such as low molecular weight, antibiotic resistance genes, an origin of replication, and several single-cut restriction endonuclease recognition sites.

Some aspects of molekulee engineering in plant and animal cells are discussed in the final section of this book. Many of the drawbacks can be overcome by using this approach, particularly when vectors that can function in the target host and skide E.

Yeast integrative plasmids YIps dirancang untuk mengintegrasi ke kromosom dalam cara yang mirip dengan plasmid YEp, Yeast replicative plasmids YRps tetap sebagai plasmid independen dan tidak mengintegrasikanYeast centromere plasmids YCps mengandung urutan dari seluruh wilayah sentromerik dari kromosom dan perilaku dasarnya sebagai minikromosom.

Plants are cheap to grow compared to the high-cost requirements of microbial or mammalian cells and, thus, cost reduction and potentially unlimited scale-up opportunities make transgenic plants an attractive option for producing high-value proteins.

In the lysogenic response the phage DNA integrates into the host genome as a prophage Pwhich can be maintained through niologi cell divisions. Presentasi saya Profil Tanggapan Keluar.

Pendahuluan Biologi Molekuler – ppt download

May be linear and composed of several segments or single and circular. The theory is simple — if plants can be made herbicide-resistant, then weeds can be treated with a broad-spectrum herbicide without the crop plant being affected.


However, widespread planting throughout Southeast Asia led to a rice monoculture, with increased susceptibility to disease and pests, and the increased dependence on pesticides that this brings. In trying to delay natural ripening, two approacheshave been used. One of themajor problems we have already mentioned has been the public acceptance of transgenic plants.

Traitor technology or GURT involves the use of a switch often controlled by biologgi chemical additive to permit or restrict a particular engineered trait. This demonstrates that the mouse molekulerr proving to be one of the mainstays of modern transgenic research. Biologo means that a cell becomes “old” and dies by a process called apoptosis.

Studi tentang perkembangan embriologis telah dikembangkan oleh kemampuan untuk memasukkan gen ke dalam telur atau embrio awal, dan ada ruang untuk manipulasi hewan ternak dengan penggabungan sifat yang diinginkan melalui transgenesis.

Can occur between prokaryotic cells or between eukaryotic cells. Biologi Molekuler – Sel Documents. Insertionand replacement phage vectors 16LO Gen nos dan ocs eukariotik dan promotornya telah digunakan secara luas dalam pembuatan vektor yang mengekspresikan urutan basa gen sisipannya.

Some success was achieved as the engineered bacteria reduced frost damage in the testtreatments. As we saw in Chapter 11, good science does not necessarily lead to a viable product, with many strategic and commercial aspects having an impact on whether or not a product is developed fully to market.

The entire genome has been sequenced this was the first major sequencing project to be completed, and represents one of the milestones of molecular geneticsand all the regulatory sites are known. The antibiotic resistance genes encoded by plasmid DNA pDNA are often used in the construction of vectors for genetic engineering, as they provide a convenient means of selecting cells containing the plasmid. For thousands of years humans have manipulated the genetic characteristics of plants by selective breeding.

Contoh ini menunjukkan janin 13 hari dari strain transgenik yang mengekspresikan transgen dalam jaringan otak selama kehamilan.